Journal: Microbial Cell Factories

Article Title: Optimization of ethylene glycol production from ( d )-xylose via a synthetic pathway implemented in Escherichia coli

doi: 10.1186/s12934-015-0312-7

Figure Lengend Snippet: Ethylene glycol production with different engineered E. coli strains expressing the synthetic pathway

Article Snippet: The Low Input Quick Amp Labeling kit (Agilent) was used to convert RNA samples to labeled cDNA which was hybridized on E. coli Gene Expression Microarrays (8 × 15 K, Agilent) following the Agilent One-Color Microarray-Based Gene Expression Analysis Protocol.

Techniques: Expressing, Mutagenesis

Journal: Microbial Cell Factories

Article Title: Optimization of ethylene glycol production from ( d )-xylose via a synthetic pathway implemented in Escherichia coli

doi: 10.1186/s12934-015-0312-7

Figure Lengend Snippet: Identification of YqhD as the major glycolaldehyde reductase in E. coli . a Production of ethylene glycol by E. coli strains depending on the deletion of candidate glycolaldehyde reductases. Cells were cultivated on mineral ( d )-xylose medium and exposed to 10 mM glycolaldehyde. Production of ethylene glycol was estimated after 10 h of incubation. b Log2 transformed expression levels of candidate glycolaldehyde reductases in wild-type cells (C1), strain Pen205 (Δ xylB expressing pEXT20-khk-C-aldoB) (C2), and wild-type cells exposed to 10 mM glycolaldehyde (C3). Genes were clustered according to the Euclidean distance between their expression levels using complete-linkage clustering . Red and blue correspond to high and low expression levels, respectively, using arbitrary units.

Article Snippet: The Low Input Quick Amp Labeling kit (Agilent) was used to convert RNA samples to labeled cDNA which was hybridized on E. coli Gene Expression Microarrays (8 × 15 K, Agilent) following the Agilent One-Color Microarray-Based Gene Expression Analysis Protocol.

Techniques: Incubation, Transformation Assay, Expressing

Journal: Microbial Cell Factories

Article Title: Optimization of ethylene glycol production from ( d )-xylose via a synthetic pathway implemented in Escherichia coli

doi: 10.1186/s12934-015-0312-7

Figure Lengend Snippet: Product yields (Y) of engineered E. coli strains expressing the synthetic pathway

Article Snippet: The Low Input Quick Amp Labeling kit (Agilent) was used to convert RNA samples to labeled cDNA which was hybridized on E. coli Gene Expression Microarrays (8 × 15 K, Agilent) following the Agilent One-Color Microarray-Based Gene Expression Analysis Protocol.

Techniques: Expressing

Journal: Microbial Cell Factories

Article Title: Optimization of ethylene glycol production from ( d )-xylose via a synthetic pathway implemented in Escherichia coli

doi: 10.1186/s12934-015-0312-7

Figure Lengend Snippet: Escherichia coli strains used in this study

Article Snippet: The Low Input Quick Amp Labeling kit (Agilent) was used to convert RNA samples to labeled cDNA which was hybridized on E. coli Gene Expression Microarrays (8 × 15 K, Agilent) following the Agilent One-Color Microarray-Based Gene Expression Analysis Protocol.

Techniques:

Journal: Journal of Clinical Laboratory Analysis

Article Title: Expression profiles of circular RNAs in colon biopsies from Crohn's disease patients by microarray analysis

doi: 10.1002/jcla.23788

Figure Lengend Snippet: Overview of the microarray results. (A) Heat map of the circRNA microarray profiles. The expression of circRNAs was hierarchically clustered on the y‐axis, and tissue samples were hierarchically clustered on the x‐axis. Green indicated lower expression levels and red presented higher expression levels. (B) The volcano plots showing differentially expressed circRNAs. The green vertical line represented twofold changes, while the horizontal line marked a p value of 0.05. (C) Scatter plots of differentially expressed circRNAs. CircRNAs above and below the border green line were expressed more than twofold changes. (D) Venn diagram summarizing differentially expressed circRNAs shared by groups. Group A: UC, Group B: CD, Group C: healthy controls

Article Snippet: Microarray hybridization was performed using a Human 8 × 15 K circRNA Array based on the Arraystar's standard protocols (Arraystar Inc.).

Techniques: Microarray, Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Expression profiles of circular RNAs in colon biopsies from Crohn's disease patients by microarray analysis

doi: 10.1002/jcla.23788

Figure Lengend Snippet: Top 10 upregulated and downregulated circRNAs in CD patients compared with UC patients screened by microarray analysis

Article Snippet: Microarray hybridization was performed using a Human 8 × 15 K circRNA Array based on the Arraystar's standard protocols (Arraystar Inc.).

Techniques: Microarray

Journal: Journal of Clinical Laboratory Analysis

Article Title: Expression profiles of circular RNAs in colon biopsies from Crohn's disease patients by microarray analysis

doi: 10.1002/jcla.23788

Figure Lengend Snippet: Top 10 upregulated and downregulated circRNAs in CD patients compared with HC screened by microarray analysis

Article Snippet: Microarray hybridization was performed using a Human 8 × 15 K circRNA Array based on the Arraystar's standard protocols (Arraystar Inc.).

Techniques: Microarray

Journal: Journal of Clinical Laboratory Analysis

Article Title: Expression profiles of circular RNAs in colon biopsies from Crohn's disease patients by microarray analysis

doi: 10.1002/jcla.23788

Figure Lengend Snippet: A snippet of detailed annotation for circRNA/miRNA/mRNAs interaction. (A) hsa_circ_0062142; (B) hsa_circ_0001666. Yellow represents circRNA, red represents miRNA, and blue represents mRNA

Article Snippet: Microarray hybridization was performed using a Human 8 × 15 K circRNA Array based on the Arraystar's standard protocols (Arraystar Inc.).

Techniques: